Monoclonal antibodies against an outer membrane protein from pathogenic Leptospira
نویسندگان
چکیده
منابع مشابه
Novel monoclonal antibodies against pathogenic Leptospira
Background Leptospirosis is a zoonotic disease caused by pathogenic bacteria belonging to the genus Leptospira and more than 1 million cases occur worldwide annually. Several mammals may carry the agent, and rats are the most important source of human infection in urban settings. The Leptospira surface proteins have an important role during pathogen infection and some of these allow the differe...
متن کاملThe rare outer membrane protein, OmpL1, of pathogenic Leptospira species is a heat-modifiable porin.
The outer membranes of invasive spirochetes contain unusually small amounts of transmembrane proteins. Pathogenic Leptospira species produce a rare 31-kDa surface protein, OmpL1, which has a deduced amino acid sequence predictive of multiple transmembrane beta-strands. Studies were conducted to characterize the structure and function of this protein. Alkali, high-salt, and urea fractionation of...
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Hybridoma-derived monoclonal antibodies were prepared against outer membrane antigens of four strains of Vibrio cholerae that were cultivated under iron-limited conditions, and these antibodies were partially characterized. We established a library of 66 hybridomas which produced monoclonal antibodies defining 16 different V. cholerae antigens. Two antigens (molecular weights, 18,000 and 112,00...
متن کاملA monoclonal antibody against a Pasteurella multocida outer membrane protein protects rabbits and mice against pasteurellosis.
Monoclonal antibodies (MAbs) directed against the 37.5-kDa outer membrane protein were produced by fusing myeloma cells with spleen cells obtained from mice immunized with a pathogenic strain of Pasteurella multocida isolated from a rabbit. Desirable MAbs were selected by enzyme-linked immunosorbent assay, whole-cell radioimmunoprecipitation (WC-RIP), and Western blot (immunoblot) analysis. WC-...
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Hybridomas secreting monoclonal antibodies specific for Pseudomonas aeruginosa outer membrane antigens were isolated. One of the antibodies was highly specific for the O antigen of the lipopolysaccharide of International Antigen Typing Scheme serotype 5 strains, reacting only weakly with a serotype 17 strain and failing to react with the outer membranes of strains representing 15 other serotype...
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ژورنال
عنوان ژورنال: Brazilian Journal of Microbiology
سال: 2003
ISSN: 1517-8382
DOI: 10.1590/s1517-83822003000500001